Mouse TROP2 / TACSTD2 Protein (His Tag)
C80403,EGP-1,GA733-1,Ly97,TROP2
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| Catalog Number | P50922-M08H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | C80403,EGP-1,GA733-1,Ly97,TROP2 |
| Molecular Weight | The secreted recombinant mouse TACSTD2 comprises 257 amino acids and has a calculated molecular mass of 29.4 kDa. As a result of glycosylation, the apparent molecular mass of rmTACSTD2 is approximately 45 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Gln 25 |
| SDS-PAGE |  |
| Purity | > 92 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain of mouse TACSTD2 (Q8BGV3?) (Met 1-Gln 270) was expressed, with a C-terminal polyhistidine tag. |
| Bio-activity | Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to TACSTD2-coated plates (40 μg/mL, 100 μL/well), > 50% cells will adhere after 1 hour at 37℃. |
| Research Area | Cell Biology |Cell Cycle |Cell division |Other cell division related protein |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | TROP-2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate. |
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