Mouse Tie2 / TEK Protein (aa 770-1122, His & GST Tag)

AA517024,Cd202b,Hyk,STK1,Tie-2,Tie2

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Catalog Number	P51087-M20B1
Organism Species	Mouse
Host	Baculovirus-Insect Cells
Synonyms	AA517024,Cd202b,Hyk,STK1,Tie-2,Tie2
Molecular Weight	The recombinant mouse TEK/GST chimera consists of 590 amino acids and has a calculated molecular mass of 68.2kDa. The recombinant protein migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 91 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mouse TEK (Q02858) (Gln770-Ala1122) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Bio-activity	1. No Kinase Activity 2. Measured by its binding ability in a functional ELISA. Immobilized mouse TEK (aa 770-1122) (P 51087-M20B1) at 2 μg/ml (100 μl/well) can bind human Ang2-Fc (P 10691-H02H) with a linear range of 0.25-2.0 μg/ml.
Research Area	Immunology |Inflammation / Inflammatory Mediator |Inflammatory Cytokines & Chemoki and Receptors |Angiopoietins and Angiopoietin-like Protein Families
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.
Reference	Jones N, et al. (1998) The Tek / Tie2 receptor signals through a novel Dok-related docking protein, Dok-R. Oncogene. 17(9): 1097-108. Sato A, et al. (1998) Characterization of TEK receptor tyrosine kinase and its ligands, Angiopoietins, in human hematopoietic progenitor cells. Int Immunol. 10(8): 1217-27. Huang L, et al. (1995) GRB2 and SH-PTP2: potentially important endothelial signaling molecules downstream of the TEK / TIE2 receptor tyrosine kinase. Oncogene. 11(10): 2097-103.