Mouse TrkB / NTRK2 Protein (His Tag)
GP145-TrkB/GP95-TrkB,Tkrb,trk-B,trkB
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| Catalog Number | P50132-M08H |
|---|---|
| Organism Species | Mouse |
| Host | Human Cells |
| Synonyms | GP145-TrkB/GP95-TrkB,Tkrb,trk-B,trkB |
| Molecular Weight | The secreted recombinant mouse NTRK2 consists of 409amino acids and has a calculated molecular mass of 46 kDa. The apparent molecular mass of rm NTRK2 is approximately 75-85 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Cys 32 |
| SDS-PAGE |  |
| Purity | > 98 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain of mouse NTRK2 (P15209-2) (Met 1-His 429) was expressed, with a C-terminal polyhistidine tag. |
| Bio-activity | 1. Measured by its binding ability in a functional ELISA. 2. Immobilized recombinant Mouse/Human BDNF Protein (Native) (P50240-MNAS) at 10 μg/ml (100 μl/well) can bind biotinylated mouse TrkB-His (P50132-M08H) with a linear range of 10-80 ng/ml. |
| Research Area | Cancer |Signal transduction |Akt Pathway |Receptor Tyrosine Kinases (RTKs) in the Akt Pathway |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) is a single transmembrane catalytic receptors with intracellular tyrosine kinase activity. TrkB/NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TrkB tyrosine kinase (TrkB) or NTRK2 is coupled to the Ras, Cdc42/Rac/RhoG, MAPK, PI3-K and PLCgamma signaling pathways. There are four members of the Trk family; TrkA, TrkB and TrkC and a related p75NTR receptor. Each family member binds different neurotrophins with varying affinities. TrkB/NTRK has highest affinity for brain-derived neurotrophic factor (BDNF) and is involved in neuronal plasticity, longterm potentiation and apoptosis of CNS neurons. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. TrkB/NTRK is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in TrkB/NTRK have been associated with obesity and mood disorders. |
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