Rat 4-1BBL / CD137L / TNFSF9 Protein (Fc Tag)
TNFSF9
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| Catalog Number | P80166-R01H |
|---|---|
| Organism Species | Rat |
| Host | Human Cells |
| Synonyms | TNFSF9 |
| Molecular Weight | The recombinant rat TNFSF9 comprises 463 amino acids and predicts a molecular mass of 50.9 kDa. The apparent molecular mass of the recombinant protein is approximately 61 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| predicted N | Glu |
| SDS-PAGE |  |
| Purity | > 96 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the rat TNFSF9 (Q80WE6) (Pro106-Gly308) was expressed, fused with Fc region of human IgG1 at the N-terminus. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized canine TNFRSF9-His (P70095-D08H) at 10 μg/ml (100 μl/well) can bind rat Fc-TNFSF9, The EC50 of rat Fc-TNFSF9 is 0.26-0.62 μg/ml. |
| Research Area | Cancer |Cancer immunology |Cytokine & Receptor |Tumor Necrosis Factor (TNF) & Receptor |TNF Receptor |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. It is shown to be a type II surface glycoprotein belonging to the TNF superfamily. Expression of 4-1BBL is restricted to APCs, such as dendritic cells, macrophages, and activated B cells. Members of the TNF-TNF receptor superfamily have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. Several studies have reported that 4-1BBL/4-1BB interaction provided a co-stimulatory signal to T cells, and increased T cell proliferation and cytokines production. In additon, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases. |
| Reference |