Rat Anti-Mouse CD4

Antibody type:Flowcytometry

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2b

Host:Rat

Application:FCM

Purify method:

Species:Mouse

Gene Name:

Synonyms:

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:6C11-E11

Dilution:

Mole Mass:

Location:

Concentration:

Sequence Similarity:

Gene Id:

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:短期内经常使用时，请将抗体2-8℃保存，至少可以保存6个月。若长期保存，请将抗体分装后-20℃至-80℃保存，切忌反复冻融。

Buffer condition:0.01M PBS,0.09% NaN3,pH7.4

Background:1. 取一定量制备好的小鼠相关细胞到流式管底；2. 取适量纯化抗体加入流式管底与细胞混匀，室温避光孵育30分钟；3. 加入2 ml PBS洗液重悬细胞以1000 rpm离心5分钟，弃上清；4. 加入适量荧光标记抗大鼠免疫球蛋白试剂，室温避光孵育20分钟;5. 加入2 ml PBS洗液重悬细胞以1000 rpm离心5分钟,弃上清;6. 加入0.3-0.5 ml PBS洗液重悬细胞，待流式细胞仪检测（样品于两小时内上机分析，若不能及时上机请固定细胞）。[PBS洗液:PBS+1%FBS+0.1%NaN3][细胞固定液：2%甲醛溶液]

Molar Function:6C11-E11识别52 kDa的小鼠CD4分子，CD4分子是一种Ⅰ型跨膜糖蛋白，表达于小鼠大多数胸腺细胞和成熟T淋巴细胞Th/i亚群细胞上。CD4分子与MHCⅡ类抗原共同在由T细胞介导的抗原识别过程中发挥作用。

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.