Rat Anti-Mouse CD4/CD8 (FITC/PE)

100ug (NP19876) Please inquiry

Antibody type:Flowcytometry

Label:

Modification:Unmodification

Isotype:IgG2b

Host:Rat

Application:FCM,IF

Purify method:

Species:Mouse

Gene Name:

Synonyms:

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

Western blot (1:100 to 1:500)
Immunofluorescence (1:50 to 1:400)
Immunohistochemistry (1:200 to 1:500)
Flow cytometry analysis (1:200 to 1:500)
Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:6C11-E11 5B11-E8

Dilution:

Mole Mass:

Location:

Concentration:

Sequence Similarity:

Gene Id:

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at 4°C. DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

Buffer condition:Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide and 0.2% (w/v) BSA

Background:1. Take 100μl a certain amount of prepared cells and add to the bottom of 5 ml tube; 2. Add 20μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;3. Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;4. Add 0.3-0.5ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined in two hours on the machine and can also be added fixation overnight at 4 ℃ then measured).[PBS wash buffer: PBS +1% FBS +0.1% NaN3;][Cell fixation: 2% formaldehyde solution]

Molar Function:

Western Blot:

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.