Rat Anti-Mouse CD8
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Antibody type:Flowcytometry
Label:Unconjugated
Modification:Unmodification
Isotype:IgG2b
Host:Rat
Application:FCM
Purify method:
Species:Mouse
Gene Name:
Synonyms:
Gene Synonyms:
Gene Full Name:
Gene Infomation:
Antigen:
Antigen Synonyms:
Clonality:Monclonal antibody
Source:
Reaction:
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:5B11-E8
Dilution:
Mole Mass:
Location:
Concentration:
Sequence Similarity:
Gene Id:
SwissProt ID:
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:短期内经常使用时,请将抗体2-8℃保存,至少可以保存6个月。若长期保存,请将抗体分装后-20℃至-80℃保存,切忌反复冻融。
Buffer condition:0.01M PBS,0.09% NaN3,pH7.4
Background:1. 取一定量制备好的小鼠相关细胞到流式管底;2. 取适量纯化抗体加入流式管底与细胞混匀,室温避光孵育30分钟;3. 加入2 ml PBS洗液重悬细胞以1000 rpm离心5分钟,弃上清;4. 加入适量荧光标记抗大鼠免疫球蛋白试剂,室温避光孵育20分钟;5. 加入2 ml PBS洗液重悬细胞以1000 rpm离心5分钟,弃上清;6. 加入0.3-0.5 ml PBS洗液重悬细胞,待流式细胞仪检测(样品于两小时内上机分析,若不能及时上机请固定细胞)。[PBS洗液:PBS+1%FBS+0.1%NaN3][细胞固定液:2%甲醛溶液]
Molar Function:5B11-E8识别小鼠CD8分子,CD8抗原表达于小鼠大多数胸腺细胞和成熟T淋巴细胞Ts/c亚群细胞上,其功能为与MHCⅠ类分子共同在抗原识别过程中发挥作用。
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.