Rat B7-DC / PD-L2 / CD273 Protein (Fc Tag)

PDCD1LG2

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Catalog Number	P80454-R02H
Organism Species	Rat
Host	Human Cells
Synonyms	PDCD1LG2
Molecular Weight	The recombinant rat PDCD1LG2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 438 amino acids and has a predicted molecular mass of 49.2 kDa. The apparent molecular mass of the protein is approximately 64.6 kDa in SDS-PAGE under reducing conditions.
predicted N	Leu 20
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the rat PDCD1LG2 (NP_001101052.2) (Met1-Arg219) was expressed with the Fc region of human IgG1 at the C-terminus.
Bio-activity	Measured by its binding ability in a functional ELISA. Immobilized rat rat PDCD1-His (P80448-R08H) at 10 μg/ml (100 μl/well) can bind rat PDCD1LG2-Fc, The EC₅₀ of rat PDCD1LG2-Fc is 6-14 ng/ml
Research Area	Immunology \|Innate Immunity \|Monocytes/Macrophages \|Co-stimulatory Molecules
Formulation	Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.
Reference	Latchman Y, et al. (2001) PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol. 2: 261-8. Carreno BM, et al. (2005) Therapeutic opportunities in the B7/CD28 family of ligands and receptors. Curr Opin Pharmacol. 5(4): 424-30. Radhakrishnan S, et al. (2007) B7-DC/PD-L2 cross-linking induces NF-kappaB-dependent protection of dendritic cells from cell death. J Immunol. 178(3): 1426-32.