Rat BTLA Protein (ECD, Fc Tag)

BTLA

• 100ug (NPP1093) Please inquiry

Catalog Number	P81142-R05H
Organism Species	Rat
Host	Human Cells
Synonyms	BTLA
Molecular Weight	The recombinant rat Btla consists 384 amino acids and predicts a molecular mass of 43.4 kDa.
predicted N	Lys 30
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE.
Protein Construction	A DNA sequence encoding the rat Btla (NP_998795.1) (Ala6-Tyr183) was expressed with the Fc region of mouse IgG1 at the C-terminus.
Bio-activity	1. Measured by its binding ability in a functional ELISA. 2. Immobilized rat BTLA-mFc at 10 μg/mL (100 μL/well) can bind mouse HVEM-Fch (P10567-M03S). The EC50 of mouse EFNA5-Fc (P50597-M02H) is 6.2-14.4 ng/mL.
Research Area	Signaling |Signal Transduction |ITIM/ITAM Immunoreceptors and Related Molecules
Formulation	Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	BTLA is a inhibitory molecule which belongs to the Ig superfamily. It down-modulates immune responses. As such, reagents that regulate the binding of BTLA to its ligand or alter BTLA signaling have significant therapeutic promise. BTLA is crucial to understand the mechanism(s) of action of these antibodies before attempting clinical applications. BTLA is not expressed by naive T cells, but it is induced during activation and remains expressed on T helper type 1 (T(H)1) but not T(H)2 cells. BTLA is a third inhibitory receptor on T lymphocytes with similarities to cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and programmed death 1 (PD-1).
Reference	Fourcade J, et al. (2012) CD8(+) T cells specific for tumor antigens can be rendered dysfunctional by the tumor microenvironment through upregulation of the inhibitory receptors BTLA and PD-1. Cancer Res. 72(4):887-96. Kojima R, et al. (2011) Molecular basis for herpesvirus entry mediator recognition by the human immune inhibitory receptor CD160 and its relationship to the cosignaling molecules BTLA and LIGHT. J Mol Biol. 413(4):762-72. Oki M, et al. (2011) A functional polymorphism in B and T lymphocyte attenuator is associated with susceptibility to rheumatoid arthritis. Clin Dev Immunol. 305656.