Rat CD14 Protein (Fc Tag)

CD14

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Catalog Number	P80308-R02H
Organism Species	Rat
Host	Human Cells
Synonyms	CD14
Molecular Weight	The recombinant rat CD14/Fc is a disulfide-linked homodimer. The reduced monomer comprises 565 amino acids and has a predicted molecular mass of 62.2 kDa. The apparent molecular mass of the protein is approximately 72 kDa in SDS-PAGE under reducing conditions.
predicted N	Ser 18
SDS-PAGE
Purity	> 93 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the rat CD14 (Q63691) (Met1-Tyr341) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Bio-activity
Research Area	Immunology |Inflammation / Inflammatory Mediator |Cells Involved in Inflammation |Myeloid-derived Suppressor Cells (MDSC)
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 14 (CD14) is a member of the CD system. It takes its name from its inclusion in the CD molecule surface marker proteins. CD14 exists in two forms: a form anchored into the membrane or a soluble form. CD14 was found expressed in macrophages, neutrophil granulocyte and dendritic cells. The major function is serve as a co-receptor (along with TLR4 and MD-2) for the bacterial lipopolysaccharide (LPS) and other pathogen-associated molecular patterns.
Reference	Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5. Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35. Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12. SD Wright, et al. (1990) CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 249 (4975): 1431-3.