Rat CD155 / PVR / NECL5 Protein (His Tag)

PVR, Taa1, Tage4

• 100ug (NPP1115) Please inquiry

Catalog Number	P80007-R08H
Organism Species	Rat
Host	Human Cells
Synonyms	PVR, Taa1, Tage4
Molecular Weight	The recombinant rat PVR comprises 330 amino acids and predicts a molecular mass of 35.8 kDa. As a result of glycosylation, the apparent molecular mass of the rat PVR is approximately 55-65 kDa in SDS-PAGE under reducing conditions.
predicted N	Glu 34
SDS-PAGE
Purity	> 97 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the extracellular domain of rat PVR (NP_058772.2) (Met 1-Gly 352) was expressed, fused with a polyhistidine tag at the C-terminus.
Bio-activity	Measured by its binding ability in a functional ELISA . Immobilized rat CD155 at 20 μg/ml (100 μl/well) can bind mouse CD226 with a linear ranger of 6.4-160 ng/ml.
Research Area	Immunology |Innate Immunity |Monocytes/Macrophages |Monocyte Markers
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	CD155, commonly known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5), is a type I transmembrane single-span glycoprotein, and belongs to the nectins and nectin-like (Necl) subfamily. CD155 was originally identified based on its ability to mediate the cell attachment and entry of poliovirus (PV), an etiologic agent of the central nervous system disease poliomyelitis. The normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155 may assist in an efficient humoral immune response generated within the intestinal immune system. It has been demonstrated that CD155 can be recognized and bond by DNAM-1 and CD96 which promote the adhension, migration and NK-cell killing, and thus efficiently prime cell-mediated tumor-specific immunity.
Reference	Freistadt MS, et al. (2000) Hematopoietic cells from CD155-transgenic mice express CD155 and support poliovirus replication ex vivo. Microb Pathog. 29(4): 203-12. Sato T, et al. (2004) Involvement of heterophilic trans-interaction of Necl-5/Tage4/PVR/CD155 with nectin-3 in formation of nectin- and cadherin-based adherens junctions. Genes Cells. 9(9): 791-9. Kakunaga S, et al. (2004) Enhancement of serum- and platelet-derived growth factor-induced cell proliferation by Necl-5/Tage4/poliovirus receptor/CD155 through the Ras-Raf-MEK-ERK signaling. J Biol Chem. 279(35): 36419-25. Sato T, et al. (2005) Common signaling pathway is used by the trans-interaction of Necl-5/Tage4/PVR/CD155 and nectin, and of nectin and nectin during the formation of cell-cell adhesion. Cancer Sci. 96(9): 578-89. Minami Y, et al. (2007) Involvement of up-regulated Necl-5/Tage4/PVR/CD155 in the loss of contact inhibition in transformed NIH3T3 cells. Biochem Biophys Res Commun. 352(4): 856-60.