Rat CD16a / FCGR3A Protein (His Tag)

Fcgr4

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Catalog Number	P80017-R08H
Organism Species	Rat
Host	Human Cells
Synonyms	Fcgr4
Molecular Weight	The recombinant rat FCGR3A comprises 190 amino acids and predicts a molecular mass of 21.7 kDa. The apparent molecular mass of the rat FCGR3A is approximately 28-33 kDa in SDS-PAGE under reducing conditions due to glycosylation.
predicted N	Leu 22
SDS-PAGE
Purity	> 97 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the rat FCGR3A (ABO70343.1) extracellular domain (Met 1-Pro 200) was expressed, fused with a polyhistidine tag at the C-terminus.
Bio-activity	Measured by its binding ability in a functional ELISA. Immobilized rat FCGR3A-His (P80017-R08H) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC₅₀ of biotinylated human IgG1 is 59.0-139.0 ng/ml.
Research Area	Immunology \|Cluster of Differentiation (CD) \|Granulocyte CD Antigen \|CD Antigen (Neutrophils)
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	David Dornan, et al. Effect of FCGR2A and FCGR3A variants on CLL outcome. Blood. 2010 116: 4212-4222 Edberg JC, et al. (2002) Genetic linkage and association of Fc gamma receptor IIIA (CD16A) on chromosome 1q23 with human systemic lupus erythematosus. Arthritis Rheum. 46(8): 2132-40. Li P, et al. (2002) Recombinant CD16A-Ig forms a homodimer and cross-blocks the ligand binding functions of neutrophil and monocyte Fcgamma receptors. Mol Immunol. 38(7): 527-38. Li P, et al. (2007) Affinity and kinetic analysis of Fcgamma receptor IIIa (CD16a) binding to IgG ligands. J Biol Chem. 282(9): 6210-21.