Rat CD34 Protein (Fc Tag)

CD34

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Catalog Number	P80262-R02H
Organism Species	Rat
Host	Human Cells
Synonyms	CD34
Molecular Weight	The recombinant rat CD34/Fc is a disulfide-linked homodimer. The reduced monomer comprises 496 amino acids and has a predicted molecular mass of 54.5 kDa. The apparent molecular mass of the protein is approximately 112 kDa in SDS-PAGE under reducing conditions.
predicted N	Glu 37
SDS-PAGE
Purity	> 80 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the rat CD34 (B1PLB1) (Met1-Thr291) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Bio-activity
Research Area	Developmental Biology |Embryogenesis |Germ Layer Formation |Mesoderm
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias.
Reference	Hogan CJ, et al. (2002) Differential long-term and multilineage engraftment potential from subfractions of human CD34+ cord blood cells transplanted into NOD/SCID mice. Proc Nat Acad Sci USA. 99 (1): 413-8. Nielsen JS,et al. (2009) CD34 is a key regulator of hematopoietic stem cell trafficking to bone marrow and mast cell progenitor trafficking in the periphery. Microcirculation. 16(6): 487-96. Mastrandrea F,et al. (2009) CD34+ hemopoietic precursor and stem cells traffic in peripheral blood of celiac patients is significantly increased but not directly related to epithelial damage severity. Eur Ann Allergy Clin Immunol. 40(3): 90-103. Pasquet S,et al. (2009) Long-term benefit of intracardiac delivery of autologous granulocyte-colony-stimulating factor-mobilized blood CD34+ cells containing cardiac progenitors on regional heart structure and function after myocardial infarct. Cytotherapy. 11(8): 1002-15.