Rat CD47 Protein (His Tag)
CD47
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| Catalog Number | P80305-R08H |
|---|---|
| Organism Species | Rat |
| Host | Human Cells |
| Synonyms | CD47 |
| Molecular Weight | The recombinant rat CD47 comprises 133 amino acids and predicts a molecular mass of 15.2 kDa. The apparent molecular mass of the recombinant protein is approximately 27-35 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| predicted N | Gln 19 |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the rat CD47 (P97829-1) (Met1-Lys140) was expressed, fused with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized Rat CD47-His at 10 μg/ml (100 μl/well) can bind mouse SIRPA-Fc (P50956-M02H), The EC50 of mouse SIRPA-Fc (P50956-M02H) is 0.6-1.4 μg/ml. |
| Research Area | Cancer |Cancer immunology |CD marker |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus. |
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