Rat CD59 / CD59A / MAC-IP Protein (Fc Tag)
CD59
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| Catalog Number | P80299-R02H |
|---|---|
| Organism Species | Rat |
| Host | Human Cells |
| Synonyms | CD59 |
| Molecular Weight | The recombinant rat CD59 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 319 amino acids and has a predicted molecular mass of 35.8 kDa. The apparent molecular mass of the protein is approximately 40 and 43 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Leu 23 |
| SDS-PAGE |  |
| Purity | (55.3+31.4) % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the rat CD59 (P27274) (Met1-Asn100) was expressed, fused with the Fc region of human IgG1 at the C-terminus. |
| Bio-activity | |
| Research Area | Cardiovascular |Atherosclerosis |Vascular Inflammation |Innate and adaptive immunity |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | CD59 glycoprotein, also known as 20 kDa homologous restriction factor, HRF20, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D). |
| Reference |