Rat CD86 / B7-2 Protein (His Tag)

B7-2

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Catalog Number	P80025-R08H
Organism Species	Rat
Host	Human Cells
Synonyms	B7-2
Molecular Weight	The recombinant rat CD86 comprises 232 amino acids and predicts a molecular mass of 26.8 kDa. The apparent molecular mass of the rat CD86 is approximately 40-70 kDa in SDS-PAGE under reducing conditions due to glycosylation.
predicted N	Pro 30
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the rat CD86 (NP_064466.1) extracellular domain (Met 1-Ile 250) was expressed, fused with a polyhistidine tag at the C-terminus.
Bio-activity	Measured by its binding ability in a functional ELISA . Immobilized rat CD86 at 2 μg/ml (100 μl/well) can bind mouse CD28 with a linear ranger of 6.4-160 ng/ml .
Research Area	Immunology |Adaptive Immunity |Regulatory T Cells
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.
Reference	Chen YQ, et al. (2006) CD28/CTLA-4--CD80/CD86 and ICOS--B7RP-1 costimulatory pathway in bronchial asthma. Allergy. 61(1): 15-26. Rau FC, et al. (2009) B7-1/2 (CD80/CD86) direct signaling to B cells enhances IgG secretion. J Immunol. 183(12): 7661-71. Dai ZS, et al. (2009) Defective expression and modulation of B7-2/CD86 on B cells in B cell chronic lymphocytic leukemia. Int J Hematol. 89(5): 656-63.