Rat EphA3 Protein (His Tag)
EPHA3
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| Catalog Number | P80465-R08H |
|---|---|
| Organism Species | Rat |
| Host | Human Cells |
| Synonyms | EPHA3 |
| Molecular Weight | The recombinant rat EPHA3 comprises 532 amino acids and predicts a molecular mass of 61.2 kDa. The apparent molecular mass of the recombinant protein is approximately 70.5 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| predicted N | Glu 21 |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the rat EPHA3 (EDL75897.1) (Met1-His541) was expressed with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized rat EPHA3-His at 10 μg/ml (100 μl /well) can bind rat EFNA5-Fc(P80105-R02H), The EC50 of ratEFNA5-Fc (P80105-R02H) is 9-20ng/ml. |
| Research Area | Cancer |Invasion microenvironment |Angiogenesis |Eph Receptors |
| Formulation | Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | EPHA3 gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA3 gene encodes a protein that binds ephrin-A ligands. EPHA3 is involved in the retinotectal mapping of neurons. It may also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development. |
| Reference |