Rat HER4 / ErbB4 Protein (His Tag)

ERBB4

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Catalog Number	P80125-R08H
Organism Species	Rat
Host	Human Cells
Synonyms	ERBB4
Molecular Weight	The recombinant rat ERBB4 comprises 637 amino acids and predicts a molecular mass of 71.3 kDa. The apparent molecular mass of the recombinant protein is approximately 75-95 kDa in SDS-PAGE under reducing conditions due to glycosylation.
predicted N	Gln 26
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the rat ERBB4 (AAQ77349.1)(Met1-Pro651) was expressed with a polyhistidine tag at the C-terminus.
Bio-activity	Measured by its binding ability in a functional ELISA. Immobilized rat ERBB4-His at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1 (P11609-H01H2), The EC₅₀ of biotinylated human NRG1 (P11609-H01H2) is 0.68-1.6 μg/ml.
Research Area	Cancer \|Invasion microenvironment \|Angiogenesis \|Growth Factor & Receptor \|Receptor Tyrosine Kinase (RTK)
Formulation	Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	ERBB4 is a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. ERBB4 is expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. And lower levels in thymus, lung, salivary gland, and pancreas. It specifically binds to and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. ERBB4 also can be activated by other factors and induces a variety of cellular responses including mitogenesis and differentiation. ERBB4 regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. It is required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. ERBB4 also play a role on the normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. It is required for mammary gland differentiation, induction of milk proteins and lactation.
Reference	Huang, Y Z, et al. (2000) Regulation of neuregulin signaling by PSD-95 interacting with ErbB4 at CNS synapses. Neuron. 26(2):443-55. Garcia, R A, et al. (2000) The neuregulin receptor ErbB-4 interacts with PDZ-containing proteins at neuronal synapses. Proc Natl Acad Sci. 97(7):3596-601. Silberberg G, et al. (2006) The involvement of ErbB4 with schizophrenia: association and expression studies. Am J Med Genet. 141(B2):142-8. Sardi SP, et al. (2006) Presenilin-dependent ErbB4 nuclear signaling regulates the timing of astrogenesis in the developing brain. Cell. 127(1):185-97.