Rat IFNGR / IFNGR1 Protein (Fc Tag)
IFNGR, Ifngr1
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| Catalog Number | P80182-R02H |
|---|---|
| Organism Species | Rat |
| Host | Human Cells |
| Synonyms | IFNGR, Ifngr1 |
| Molecular Weight | The recombinant rat IFNGR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 466 amino acids and has a predicted molecular mass of 52.4 kDa. The apparent molecular mass of the protein is approximately 117 and 65-80 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Ala 17 |
| SDS-PAGE |  |
| Purity | (22.7+76.3) % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the rat IFNGR (Q6P6T3) (Met1-Ser241) was expressed with the Fc region of human IgG1 at the C-terminus. |
| Bio-activity | 1. Measured by its binding ability in a functional ELISA. Immobilized rat IFNGR-Fc (cat:80182-R02H) at 10 μg/ml (100 μl/well) can bind biotinylated rat IFNG-Fc (P80234-R02H). The EC50 of biotinylated rat IFNG-Fc (P80234-R02H）is 0.10-0.24 μg/ml. 2. Measured by its ability to inhibit rat IFNγ-mediated protection of L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 0.5-4 ng/mL in the presence of 10 ng/mL of recombinant rat IFN-gamma. |
| Research Area | Cardiovascular |Angiogenesis |Cytokine & Receptor |Interferon & Receptor |Interferon receptor |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD119 (cluster of differentiation 119), also known as IFNGR1 ( interferon gamma receptor 1), is part of the heterodimeric gamma interferon receptor which consists of IFNGR1 (CD119) and IFNGR2. The IFNGR1 gene encodes the ligand-binding chain (alpha) of the interteron receptor while IFNGR gene encodes the non-ligand binding partner. The ability of the interferon-γ was achieved through binding to the interferon receptor CD119. After binding, the products of activated T-lymphocytes interferon-γ exerts antiviral activity, growth inhibitory effect, and several immune- regulatory activities on a variety of cell types. |
| Reference |