Rat IL-21R / Interleukin-21 Receptor Protein (His Tag)
IL21R
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| Catalog Number | P80189-R08H |
|---|---|
| Organism Species | Rat |
| Host | Human Cells |
| Synonyms | IL21R |
| Molecular Weight | The recombinant rat IL21R comprises 228 amino acids and predicts a molecular mass of 26.5 kDa. The apparent molecular mass of the recombinant protein is approximately 43-48 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| predicted N | Cys 20 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the rat IL21R (Q5EBB1) (Met1-Pro236) was expressed, fused with a polyhistidine tag at the C-terminus. |
| Bio-activity | Immobilized rat IL21R-His at 10 μg/ml (100 μl/well) can bind biotinylated rat IL21-His (P80200-R08B), The EC50 of biotinylated rat IL21-His (P80200-R08B) is 4.6-10.9 ng/ml. |
| Research Area | Immunology |Cytokines & Growth Factors |Cytokine & Receptor |Interleukin & Receptor |Other Interleukin & Receptor |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Interleukin-21 receptor, also known as IL-21 receptor, IL-21R, Novel interleukin receptor, IL21R and NILR, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 4 subfamily. Interleukin-21 (IL-21) belongs to a family of cytokines that bind to a composite receptor consisting of a private receptor (IL-21R) and the common cytokine receptor gamma chain ( gamma(C) ). The IL-21R is discovered as a novel member of the class-I-cytokine-receptor family and is selectively expressed in lymphoid tissues. IL-21R shows strong sequence homologies to the interleukin-4 receptor alpha chain gene (IL-4RA). The WSXWS motif of IL-21R appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif of IL-21R is required for JAK interaction and / or activation. The IL-21R is widely distributed on lympho-haematopoietic cells and IL21 impacts a number of cell types, including CD8+ memory T cells, NK cells and subsets of CD4 memory T cells. Increased IL21 production is characteristic of certain autoimmune diseases and is likely to contribute to autoantibody production as well as pathological features of autoimmune disease. The critical role of IL21 in promoting humoral immune responses makes it an important focus of potential therapeutic interventions in conditions characterised by overproduction of pathogenic autoantibodies. |
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