Rat IL17F / IL-17F Protein (His Tag)

IL17F, IL-17F

100ug (NPP3061) Please inquiry

Catalog Number	P80462-R08B
Organism Species	Rat
Host	Baculovirus-Insect Cells
Synonyms	IL17F, IL-17F
Molecular Weight	The recombinant rat Il17f consists 145 amino acids and predicts a molecular mass of 16.4 kDa.
predicted N	Ala 19
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE.
Protein Construction	A DNA sequence encoding the rat Il17f (NP_001015011.1) (Met1-Ala153) was expressed with a polyhistidine tag at the C-terminus.
Bio-activity	1. Measured by its binding ability in a functional ELISA. 2. Immobilized rat IL17F-His at 10 μg/mL (100 μL/well) can bind Rat IL17RA-Fc3 (P80190-R02H). The EC₅₀ of Rat IL17RA-Fc3 (P80190-R02H) is 0.28-0.66 μg/mL.
Research Area	Immunology \|Innate Immunity \|Cytokine \|Interleukins
Formulation	Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Interleukin-17F (IL-17F) is a cytokine that shares sequence similarity with IL17. The most notable role of IL-17 is it involvement in inducing and mediating proinflammatory responses. IL-17 is commonly associated with allergic responses. IL-17F is expressed by activated T cells, and was expressed only in activated CD4+ T cells and activated monocytes. IL-17F has been shown to stimulate the production of several other cytokines, including IL6 and IL8. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. Recombinant human IL-17F did not stimulate the proliferation of hematopoietic progenitors or the migration of mature leukocytes. However, it markedly inhibited the angiogenesis of human endothelial cells and induced endothelial cells to produce IL-2, TGF-{beta}, and monocyte chemoattractant protein-1. IL-17F stimulates the production of other cytokines and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. IL-17F stimulates PBMC and T-cell proliferation. It also function in inhibiting angiogenesis By similarity. IL-17F plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.
Reference	et al.. Starnes T, et al.. (2001) Cutting edge: IL-17F, a novel cytokine selectively expressed in activated T cells and monocytes, regulates angiogenesis and endothelial cell cytokine production. J Immunol. 167(8): 4137-40. Hymowitz SG, et al.. (2001) IL-17s adopt a cystine knot fold: structure and activity of a novel cytokine, IL-17F, and implications for receptor binding. EMBO J. 20(19): 5332-41. McAllister F, et al.. (2005) Role of IL-17A, IL-17F, and the IL-17 receptor in regulating growth-related oncogene-alpha and granulocyte colony-stimulating factor in bronchial epithelium: implications for airway inflammation in cystic fibrosis. J Immunol. 175(1): 404-12.