Rat IL1R1 / CD121a Protein (His & Fc Tag)
Il1r1
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| Catalog Number | P80028-R06H |
|---|---|
| Organism Species | Rat |
| Host | Human Cells |
| Synonyms | Il1r1 |
| Molecular Weight | The recombinant rat IL1R1/mFc is a disulfide-linked homodimer. The reduced monomer comprises 562 amino acids and predicts a molecular mass of 64.6 kDa. The apparent molecular mass of the ratIL1R1/mFc monomer is approximately 90-95 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| predicted N | Leu 34 |
| SDS-PAGE |  |
| Purity | > 85 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the rat IL1R1 (NP_037255.3) extracellular domain (Met 1-Lys 352) was was fused with the C-terminal His-tagged Fc region of mouse IgG1 at the C-terminus. |
| Bio-activity | Measured by its ability to inhibit IL1α-dependent proliferation in D10.G4.1 mouse helper T cells（Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.). The ED50 for this effect is typically 5-20 μg/ml in the presence of 40 pg/mL of recombinant human IL1a. |
| Research Area | Immunology |Inflammation / Inflammatory Mediator |Inflammatory Disorders Therapeutic Targets |Rheumatoid arthritis Therapeutic Targets |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA). IL-1R1/CD121a is an important mediator involved in many cytokine induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1β activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1β induced effects. In the spleen, a slight increase in IL-1R AcP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess. |
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