Rat IL21 / Interleukin 21 Protein (His Tag)

IL21

• 100ug (NPP1755) Please inquiry

Catalog Number	P80200-R08B
Organism Species	Rat
Host	Baculovirus-Insect Cells
Synonyms	IL21
Molecular Weight	The recombinant rat IL21 consists of 139 amino acids and predicts a molecular mass of 16.6 KDa. It migrates as an approximately 20 KDa band in SDS-PAGE under reducing conditions.
predicted N	His 18
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the rat IL21 (NP_001102413.1) (Met1-Ser146) was expressed with a polyhistide tag at the C-terminus.
Bio-activity	Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 2-8 ng/ml.
Research Area	Immunology |Inflammation / Inflammatory Mediator |Inflammatory Cytokines & Chemoki and Receptors |Common gamma Chain Receptor Family
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4. 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Coquet JM, et al. (2007) IL-21 is produced by NKT cells and modulates NKT cell activation and cytokine production. J Immunol. 178(5):2827-34. Wei L, et al. (2007) IL-21 is produced by Th17 cells and drives IL-17 production in a STAT3-dependent manner. J Biol Chem. 282(48):34605-10. Parrish-Novak J, et al. (2002) Interleukin-21 and the IL-21 receptor: novel effectors of NK and T cell responses. J Leukoc Biol. 72(5):856-63. 4 Kuchen S, et al. (2007) Essential role of IL-21 in B cell activation, expansion, and plasma cell generation during CD4+ T cell-B cell collaboration. J Immunol. 179(9):5886-96.