Rat IL3 / interleukin 3 Protein (His Tag)
Il3
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| Catalog Number | P80235-R08B |
|---|---|
| Organism Species | Rat |
| Host | Baculovirus-Insect Cells |
| Synonyms | Il3 |
| Molecular Weight | The secreted recombinant rat IL3 comprises 153 amino acids with and has a predicted molecular mass of 17.5 kDa. It migrates as an approximately 24 kDa band in reduced SDS-PAGE. |
| predicted N | Ile 27 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the rat IL3 (NP_113701.1) (Met 1-Cys 169) was fused with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured in a cell proliferation assay using FDC-P1 cells. The ED50 for this effect is typically 2-8 ng/mL. |
| Research Area | Immunology |Cytokines & Growth Factors |Cytokine & Receptor |Interleukin & Receptor |Other Interleukin & Receptor |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.0, 10% gly 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | IL3 (interleukin 3), also known as IL-3, is a potent growth promoting cytokine which belongs to the IL-3 family. IL3/IL-3 also belongs to the group of interleukins. Interleukins are produced by a wide variety of body cells. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4+ T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T, B, and hematopoietic cells. IL3/IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL3/IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. |
| Reference |