Rat SIRP alpha / CD172a Protein (His Tag)
SIRPA, Bit, Mfr, Ptpns1, Shps1, Sirp
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| Catalog Number | P80270-R08H |
|---|---|
| Organism Species | Rat |
| Host | Human Cells |
| Synonyms | SIRPA, Bit, Mfr, Ptpns1, Shps1, Sirp |
| Molecular Weight | The recombinant rat SIRPA comprises 353 amino acids and predicts a molecular mass of 39 kDa. The apparent molecular mass of the recombinant protein is approximately 63-80 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| predicted N | Lys 32 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the rat SIRPA (P97710) (Met1-Asn373) was expressed with a polyhistidine tag at the C-terminus. |
| Bio-activity | |
| Research Area | Neuroscience |Neurology process |Neural Signal Transduction |
| Formulation | Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. |
| Reference |