Rat TNFR1 / CD120a / TNFRSF1A Protein (Fc Tag)

TNFRSF1A, Tnfr-1, Tnfr1

100ug (NPP3140) Please inquiry

Catalog Number	P80181-R02H
Organism Species	Rat
Host	Human Cells
Synonyms	TNFRSF1A, Tnfr-1, Tnfr1
Molecular Weight	The recombinant rat TNFRSF1A/Fc is a disulfide-linked homodimer. The reduced monomer comprises 431 amino acids and has a predicted molecular mass of 47.9 kDa. The apparent molecular mass of the protein is approximately 59 kDa in SDS-PAGE under reducing conditions.
predicted N	Ile 22
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the rat TNFRSF1A (P22934) (Met1-Ala211) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Bio-activity	1. Measured by its ability to inhibit TNFα-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of metabolic inhibitor actinomycin D. The ED₅₀ for this effect is typically 3-30 ng/mL in the presence of 0.05 ng/mL of ratTNFα. 2. Immobilized mouse TNFa (80-235) (P50349-MNAE ) at 10 μg/ml (100 μl/well) can bind rat TNFRSF1A-Fc, The EC₅₀ of rat TNFRSF1A-Fc is 0.08-0.20 μg/ml.
Research Area	Developmental Biology \|Metabolism \|Pathways and Processes \|Metabolism processes \|Apoptosis \|Intracellular \|
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.
Reference	Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5. Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35. Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12. Cottin V, et al. (2002) Restricted localization of the TNF receptor CD120a to lipid rafts: a novel role for the death domain. The journal of immunology. 168: 4095-102.