Rat VEGFR1 / FLT-1 Protein (His Tag)
FLT1, Flt-1, Vegfr1
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| Catalog Number | P80101-R08H |
|---|---|
| Organism Species | Rat |
| Host | Human Cells |
| Synonyms | FLT1, Flt-1, Vegfr1 |
| Molecular Weight | The recombinant rat FLT1 comprises 747 amino acids and predicts a molecular mass of 84 kDa. The apparent molecular mass of the recombinant protein is approximately 108-114 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| predicted N | Tyr 23 |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the rat FLT1 (P53767) (Met1-Glu758) was expressed with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized ratFLT1-His (P 80101-R08H) at 10 μg/ml (100 μl/well) can bind biotinylated mouse VEGFD-Fc (P 50157-M01H) with a linear range of 0.125-2.0 μg/ml. |
| Research Area | Signaling |Signal Transduction |Protein Kinase |Receptor Tyrosine Kinase (RTK) |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Vascular endothelial growth factor receptor 1, also known as VEGFR-1, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular permeability factor receptor and FLT1, is a single-pass type I membrane protein and secreted protein which belongs to the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR-1 / FLT1 contains seven Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. VEGFR-1 / FLT1 is expressed mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. It is specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. VEGFR-1 / FLT1 is not expressed in tumor cell lines. VEGFR-1 / FLT1 is an essential receptor tyrosine kinase that regulates mammalian vascular development and embryogenesis. EGF-induced angiogenesis requires inverse regulation of VEGFR-1 and VEGFR-2 in tumor-associated endothelial cells. VEGFR-1 / FLT1 is a receptor for VEGF, VEGFB and PGF. It has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. |
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