Rat c-MET / HGFR Protein (Fc Tag)
MET, Capza2, Cav , Cav2 , Cftr , LOC500040 , ST7 , Wnt2
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| Catalog Number | P80004-R02H |
|---|---|
| Organism Species | Rat |
| Host | Human Cells |
| Synonyms | MET, Capza2, Cav , Cav2 , Cftr , LOC500040 , ST7 , Wnt2 |
| Molecular Weight | The recombinant rat MET/Fc is a disulfide-linked homodimer. The reduced monomer comprises 1149 amino acids and has a predicted molecular mass of 128.1 kDa. The apparent molecular mass of the protein is approximately 97 and 39 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Glu 25 |
| SDS-PAGE |  |
| Purity | (63.0+32.1) % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the rat MET (Q2IBC7) (Met1-Asn932) was expressed with the Fc region of human IgG1 at the C-terminus. |
| Bio-activity | Immobilized rat HGF (P80429-RNAH) at 10 μg/ml (100 μl/well) can bind Rat MET-Fc, EC50 of Rat MET-Fc is 0.02-0.05 μg/ml. |
| Research Area | Cancer |Signal transduction |Protein Phosphorylation |Tyrosine Kinase |Receptor Tyrosine Kinases |
| Formulation | Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer. |
| Reference |