Rhesus CD16 / FCGR3 Protein (His & AVI Tag)

FCGR3, CD16

• 100ug (NPP2894) Please inquiry

Catalog Number	P90013-C27H
Organism Species	Rhesus
Host	Human Cells
Synonyms	FCGR3, CD16
Molecular Weight	The recombinant rhesus CD16 consists of 223 amino acids and has a calculated molecular mass of 25.3 kDa.
predicted N	Gly 17
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the rhesus CD16 (NP_001258584.1) extracellular domain (Met 1- Gln 208) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus.
Bio-activity	Measured by its binding ability in a functional ELISA. Immobilized Rhesus CD16-AVI-His at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1The EC50 of biotinylated human IgG1 is 0.2-0.5 μg/ml.
Research Area	Signaling |Signal Transduction |ITIM/ITAM Immunoreceptors and Related Molecules
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.
Reference	Edberg JC, et al. (2002) Genetic linkage and association of Fcgamma receptor IIIA (CD16A) on chromosome 1q23 with human systemic lupus erythematosus. Arthritis Rheum. 46(8): 2132-40. Li P, et al. (2002) Recombinant CD16A-Ig forms a homodimer and cross-blocks the ligand binding functions of neutrophil and monocyte Fcgamma receptors. Mol Immunol. 38(7): 527-38. Li P, et al. (2007) Affinity and kinetic analysis of Fcgamma receptor IIIa (CD16a) binding to IgG ligands. J Biol Chem. 282(9): 6210-21.