Rhesus CD27 / TNFRSF7 Protein (Fc Tag)

CD27

100ug (NPP1133) Please inquiry

Catalog Number	P90049-C02H
Organism Species	Rhesus
Host	Human Cells
Synonyms	CD27
Molecular Weight	The recombinant rhesus comprises 413 amino acids and has a calculated molecular mass of 46.3 KDa.
predicted N	Thr 21
SDS-PAGE
Purity	(87.6+8.4) % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the rhesus CD27 (XP_001104337.1) (Met1-Ile192) was expressed with the Fc region of human IgG1 at the C-terminus.
Bio-activity	Immobilized Rhesus CD27-Fc at 10 μg/ml (100 μl/well) can bind biotinylated human CD70-Fc (P10780-H01H), The EC₅₀ of biotinylated human CD70-Fc (P10780-H01H) is 10.9-25.3 ng/ml.
Research Area	Cancer \|Signal transduction \|Other Related Intracellular Topics \|Regulation of Apoptosis by TNF Superfamily Members
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.
Reference	Drner T, et al. (2004) Correlation of circulating CD27 high plasma cells and disease activity in systemic lupus erythematosus. Lupus. 13(5): 283-9. Sahota SS, et al. (2009) CD27 in defining memory B-cell origins in Waldenstrm's macroglobulinemia. Clin Lymphoma Myeloma. 9(1): 33-5. Jiang J, et al. (2010) Reduced CD27 expression on antigen-specific CD4+ T cells correlates with persistent active tuberculosis. J Clin Immunol. 30(4): 566-73.