Rhesus DDR2 Kinase / CD167b Protein (Fc Tag)
DDR2
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Catalog Number | P90228-C02H |
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Organism Species | Rhesus |
Host | Human Cells |
Synonyms | DDR2 |
Molecular Weight | The recombinant rhesus DDR2 comprises 619 amino acids and has a calculated molecular mass of 69.7 KDa. |
predicted N | Lys 22 |
SDS-PAGE | |
Purity | > 95 % as determined by SDS-PAGE |
Protein Construction | A DNA sequence encoding the rhesus DDR2 (XP_001118219.1) (Met1-Arg399) was expressed with the Fc region of human IgG1 at the C-terminus. |
Bio-activity | 1. Measured by its binding ability in a functional ELISA. 2. Immobilized Rat tail Collagen I at 10 μg/mL (100 μL/well) can bind Rhesus DDR2-Fc (P90228-C02H). The EC50 of Rhesus DDR2-Fc (P90228-C02H) is 0.23-0.55μg/mL. |
Research Area | Signaling |Signal Transduction |Receptor Tyrosine Kinases (RTKs) |
Formulation | Lyophilized from sterile PBS, PH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
Background | Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity. |
Reference | et al. |