Rhesus IFNG / Interferon Gamma Protein

IFNG

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Catalog Number	P90008-CNAE
Organism Species	Rhesus
Host	E. coli
Synonyms	IFNG
Molecular Weight	The recombinant rhesus IFNG consists of 143 amino acids and has a calculated molecular mass of 16.9 kDa.
predicted N	Met
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the rhesus IFNG (NP_001028077.1) (Gln24-Gln165) was expressed and purified with an initial Met.
Bio-activity	Measured by its binding ability in a functional ELISA. Immobilized Rhesus IFNG (cat：90008-CNAE）at 10 μg/ml (100 μl/well) can bind Rhesus IFNGR1-Fc (P90207-C02H). The EC₅₀ of Rhesus IFNGR1-Fc (P90207-C02H) is 20.28-47.32 ng/ml.
Research Area	Cancer \|Invasion microenvironment \|Angiogenesis \|Cytokine & Receptor \|Interferon & Receptor \|Interferon \|
Formulation	Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	IFN gamma, also known as IFNG, is a secreted protein which belongs to the type I I interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.
Reference	Gray P W, et al. (1982) Structure of the human immune interferon gene. Nature. 298: 859-63. Taya Y, et al. (1982) Cloning and structure of the human immune interferon-gamma chromosomal gene. EMBO J. 1: 953-8. Goshima N, et al. (2008) Human protein factory for converting the transcriptome into an in vitro-expressed proteome. Nomura N Nat Methods. 5: 1011-7. Thiel DJ, et al. (2000) Observation of an unexpected third receptor molecule in the crystal structure of human interferon-gamma receptor complex. Structure. 8 (9): 927-36. Naylor SL, et al. (1983) Human immune interferon gene is located on chromosome 12. J Exp Med. 157 (3): 1020-7. Schoenborn JR, et al. (2007) Regulation of interferon-gamma during innate and adaptive immune responses. Adv Immunol. 96: 41-101.