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Rhesus IL-12 (IL12A & IL12B Heterodimer) Protein

IL12A & IL12B

Catalog Number PCT045-C08H
Organism Species Rhesus
Host Human Cells
Synonyms IL12A & IL12B
Molecular Weight The recombinant rhesus IL12A & the rhesus IL12B comprises 525(208+317) amino acids and has a calculated molecular mass of 60.2(24+36.2) KDa.
predicted N Arg 23 & Ile 23
SDS-PAGE
Purity (23.1+26.7+49.4) % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the rhesus IL12A (NP_001038199.1) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the rhesus IL12B (NP_001038190.1) (Met1-Ser328) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the rhesus IL12A & the rhesus IL12B heterodimer was purified.
Bio-activity
Research Area Immunology |Cytokines & Growth Factors |Cytokine & Receptor |Interleukin & Receptor |IL-12 family & Receptor |IL-12 family ligand |
Formulation Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference