Rhesus IL10RA Protein (Fc Tag)

IL10RA

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Catalog Number	P90125-C02H
Organism Species	Rhesus
Host	Human Cells
Synonyms	IL10RA
Molecular Weight	The recombinant rhesus IL10RA comprises 458 amino acids and has a calculated molecular mass of 51.8 KDa.
predicted N	Phe 29
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the rhesus IL10RA (XP_001092736.1) (Thr9-Asn245) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Bio-activity	Measured by its binding ability in a functional ELISA. Immobilized Rhesus IL10RA-Fc (cat:90125-C02H) at 10 μg/ml (100 μl/well) can bind biotinylated human IL10 (P10947-H07H), The EC50 of biotinylated human IL10 (P10947-H07H) is 39.0-92.0 ng/ml.
Research Area	Neuroscience |Neurology process |Neuroinflammation
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	CD219, also known as IL10RA, is a receptor for interleukin 10. CD219 has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. It is structurally related to IFN receptors. It has been reported that CD219 promotes survival of progenitor myeloid cells. Activation of CD219 leads to tyrosine phosphorylation of JAK1 and TYK2 kinases.
Reference	Josephson K, et al. (2001) Purification, crystallization and preliminary X-ray diffraction of a complex between IL-10 and soluble IL-10R1. Acta Crystallogr D Biol Crystallogr. 57(Pt 12): 1908-11. Tan, J C, et al. (1995) Characterization of recombinant extracellular domain of human interleukin-10 receptor. J Biol Chem. 270(21):12906-11. Josephson, K, et al. (2001) Crystal structure of the IL-10/IL-10R1 complex reveals a shared receptor binding site. Immunity. 15(1):35-46.